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Microscopy (Oxford, England) Dec 2018Although conventional toluidine blue staining is a common technique used for rapid observation of semithin sections prior to transmission electron microscopy, it is...
Although conventional toluidine blue staining is a common technique used for rapid observation of semithin sections prior to transmission electron microscopy, it is monochromatic and insufficient for accurate identification of different tissue components by light microscopy. Additionally, polychromatic staining methods generally require step-by-step processes involving different dyes, and it is often difficult to balance the color tone of each step. In this study, we developed a simple polychromatic staining method for epoxy-embedded tissue sections. We stained preheated sections with an aqueous ethanol solution of azure B and basic fuchsin, with the addition of sodium tetraborate to enhance the staining efficacy. We optimized various staining conditions to enable sufficient coloration easily and consistently in a single, rapid staining step, using a single staining-mixture solution. Our method enabled clear differentiation of various tissue structures according to color tone and stain intensity, thereby facilitating the detection of fine structural differences, including various organelle and inclusion bodies. This technique represents a simple polychrome-staining method to allow more informative and convincing histological investigation in various fields of research and education.
Topics: Animals; Azure Stains; Bone and Bones; Epoxy Resins; Histological Techniques; Mice; Mice, Inbred BALB C; Mice, Inbred C57BL; Microscopy; Rosaniline Dyes; Skin; Specimen Handling; Staining and Labeling
PubMed: 30321369
DOI: 10.1093/jmicro/dfy037 -
ACS Omega Dec 2019This paper deals with the innovation of the Czech colorimetric biosensor Detehit designed for the simple, fast, and sensitive detection of nerve agents. The innovation...
This paper deals with the innovation of the Czech colorimetric biosensor Detehit designed for the simple, fast, and sensitive detection of nerve agents. The innovation is based on the use of an indicator consisting of a mixture of two triphenylmethane dyes, Guinea green B and a basic fuchsin, on a glass nanofiber filter paper carrier. The advantage of this solution is the blue-red color transition, which is much more visible than the white-yellow transition of other Detehit biosensors. The newly designed biosensor allows the users to visually detect (with the naked eye) the presence of the most significant paralytic substances (sarin, soman, cyclosarin, tabun, VX) in water at concentrations of at least 0.001 μg/mL. This biosensor design also enables one to detect these substances in air or on contaminated surfaces.
PubMed: 31867489
DOI: 10.1021/acsomega.9b02153 -
Nanomaterials (Basel, Switzerland) Jan 2022To achieve the full utilization of waste rice noodle (WRN) without secondary pollution, activated carbon (AC) and carbon quantum dots/titanium dioxide (CQDs/TiO)...
To achieve the full utilization of waste rice noodle (WRN) without secondary pollution, activated carbon (AC) and carbon quantum dots/titanium dioxide (CQDs/TiO) composite were simultaneously synthesized by using WRN as raw material. Both of the two materials showed potential applications in water pollution control. The AC based on WRN displayed a porous spherical micro-morphology, which could absorb heavy metal elements like Pb(II) and Cr(VI) efficiently, with a maximum equilibrium uptake of 12.08 mg·g for Pb(II) and 9.36 mg·g for Cr(VI), respectively. The adsorption of the resulted AC could match the Freundlich adsorption isotherm and the pseudo-second-order kinetics mode. On the other hand, the CQDs/TiO composite based on WRN displayed a high efficient photocatalytic degradation effect on various water-soluble dyes such as methylene blue, malachite green, methyl violet, basic fuchsin, and rhodamine B under visible light irradiation, which showed better photocatalytic performance than commercial TiO. The introduction of CQDs based on WRN to TiO could result in efficient electron-hole pair separation and enable more photogenerated electrons to reduce O and more photogenerated holes to oxidize HO or OH, which could cause stronger abilities in producing O and ·OH radical and better photocatalytic activity.
PubMed: 35159817
DOI: 10.3390/nano12030472 -
AJNR. American Journal of Neuroradiology Apr 2015The development of new generation flow-diverting devices will improve the result of flow diversion in challenging aneurysms. The Flow-Redirection Endoluminal Device...
BACKGROUND AND PURPOSE
The development of new generation flow-diverting devices will improve the result of flow diversion in challenging aneurysms. The Flow-Redirection Endoluminal Device system is a dual-layer flow-diversion device. The purpose of this study was to evaluate the effectiveness and safety of the Flow-Redirection Endoluminal Device in a sidewall aneurysm model and in the abdominal aorta in rabbits.
MATERIALS AND METHODS
Single Flow-Redirection Endoluminal Devices were implanted in the right common carotid artery across sidewall, vein-pouch aneurysms and within the abdominal aorta in 22 New Zealand white rabbits and followed for 1 (n = 5), 3 (n = 5), 6 (n = 4), and 12 months (n = 8). Aneurysm occlusion was graded on a 3-point scale based on digital subtraction angiography (grade I, complete occlusion; grade II, near-complete occlusion; and grade III, incomplete occlusion). Toluidine blue and basic fuchsin staining was used for the evaluation of thrombus organization within the aneurysm and neck coverage with neointima. A scanning electron microscope was used for confirmation of the patency of branch vessels along with DSA.
RESULTS
Grades I and II occlusion rates were noted in 19 (86%) and 3 (14%) aneurysms, respectively, which indicated a 100% rate of complete or near-complete occlusion. No parent artery and branch artery occlusion was shown on DSA. Histologic images indicated partial or complete intraluminal thrombus organization and neointima coverage across the aneurysm neck. A scanning electron microscope indicated that all the vessel branches along the length of the device remained patent.
CONCLUSIONS
The Flow-Redirection Endoluminal Device in experimental aneurysms demonstrated high rates of progressive and complete aneurysm occlusion while preserving the patency of branch vessels.
Topics: Animals; Aortic Aneurysm, Abdominal; Disease Models, Animal; Prostheses and Implants; Rabbits
PubMed: 25414000
DOI: 10.3174/ajnr.A4167 -
Nanomaterials (Basel, Switzerland) Aug 2022The electro-Fenton (EF) process is regarded as an efficient and promising sewage disposal technique for sustainable water environment protection. However, current...
The electro-Fenton (EF) process is regarded as an efficient and promising sewage disposal technique for sustainable water environment protection. However, current developments in EF are largely restricted by cathode electrocatalysts. Herein, a supramolecular self-assembly strategy is adopted for synthetization, based on melamine-cyanuric acid (MCA) supramolecular aggregates integrated with carbon fixation using 5-aminosalicylic acid and zinc acetylacetonate hydrate. The prepared carbon materials characterize an ordered lamellar microstructure, high specific surface area (595 m g), broad mesoporous distribution (4~33 nm) and high N doping (19.62%). Such features result from the intrinsic superiority of hydrogen-bonded MCA supramolecular aggregates via the specific molecular assembly process. Accordingly, noteworthy activity and selectivity of HO production (~190.0 mg L with 2 h) are achieved. Excellent mineralization is declared for optimized carbon material in several organic pollutants, namely, basic fuchsin, chloramphenicol, phenol and several mixed triphenylmethane-type dyestuffs, with total organic carbon removal of 87.5%, 74.8%, 55.7% and 54.2% within 8 h, respectively. This work offers a valuable insight into facilitating the application of supramolecular-derived carbon materials for extensive EF degradation.
PubMed: 36014686
DOI: 10.3390/nano12162821 -
World Journal of Orthopedics Sep 2017To develop methods of articular cartilage preparation for X-ray-electron probe microanalysis and to study its elements content in experimental osteoarthrosis.
AIM
To develop methods of articular cartilage preparation for X-ray-electron probe microanalysis and to study its elements content in experimental osteoarthrosis.
METHODS
Twenty dogs aged 2-8 years were divided in research (aged 2 years, induction of osteoarthrosis - IOA) and intact group. Intact group included three subgroups (aged 2, 5 and 8 years). Samples of cartilage after araldite saturation and pouring were partially cut into semithin sections stained with methylene blue and with methylene blue-basic fuchsin. Their smooth surfaces were investigated by X-ray-electron probe microanalysis. Spatial distribution of sulfur, calcium and phosphorus and their concentrations (weight %) were investigated.
RESULTS
X-ray electron probe microanalysis revealed non-uniform sulfur distribution in cartilage of intact animals: Its content increases from superficial zone to deep one, this regularity was preserved in animals with IOA. Differences of IOA with spontaneous chondropathy were revealed. Spontaneous aging was characterized by calcium and phosphorus storage in deep and calcified zones and compensatory increase of sulfated glycosaminoglycans in intermediate and deep cartilage zones as evidenced by the metachromatic reaction and microanalysis data. Unlike spontaneous chondropathy connected with aging in experimentally stimulated osteoarthrosis more intensive storage of calcium but minor phosphorus in intermediate zone were marked. In IOA the calcified cartilage thinning and osteoclastic resorption are apparent with few changes of elements composition; the only difference from control is minority phosphorus content.
CONCLUSION
The obtained results demonstrate specific tricks of X-ray electron probe microanalysis and its possibility in the research of mechanisms of articular cartilage alterations in osteoarthrosis.
PubMed: 28979851
DOI: 10.5312/wjo.v8.i9.681 -
Acta Medica Academica Nov 2013The aim of this study was to evaluate the effects of different techniques of surface treatment on the microleakage of fissure sealants in permanent molar teeth in vitro. (Review)
Review
OBJECTIVE
The aim of this study was to evaluate the effects of different techniques of surface treatment on the microleakage of fissure sealants in permanent molar teeth in vitro.
MATERIALS AND METHODS
96 extracted impacted human third molars were randomly divided into 8 surface treatment groups (n=12/group) as 1. Er: YAG laser ; (Fidelis II, Fotona, Ljubljana, Slovenia) (125 mj, 20Hz) ; 2. Er: YAG laser + 37% H3PO4 (15s) ; 3. ER: AG laser + 37% H3PO4+Prime&Bond NT ; 4. Er: YAG laser + G Bond ; 5. Er: YAG laser + Prime&Bond NT ; 6. 37% H3PO4 ; 7. 37% H3PO4 + Prime&Bond NT ; 8. G Bond. Sealant material (Clinpro, 3M ESPE, Seefeld, Germany), was applied into the fissures and light-cured for 20s with LED (Bluephase C5, Ivoclar-Vivadent, Schaan, Liechtenstein). Specimens were subjected to thermocycling (1000×, 5-55°C, dwell time: 15s) and immersed in 0.5% basic fuchsin solution for 24h at 37°C. The samples were sectioned and scored on a 3 point rating scale using a light microscope with a magnification of ×20. One-way analysis of variance was used to analyze data. Multiple comparisons were analyzed using Bonferroni test (p=0.05).
RESULTS
Er:YAG laser showed the highest microleakage scores whereas Er YAG laser + 37% H3PO4 showed the lowest. Although 37% H3PO4 group showed higher scores than Er:YAG laser + 37% H3PO4, the difference was not statistically significant.
CONCLUSION
Etching fissures with phosphoric acid is sufficient prior to fissure sealant application.
Topics: Acid Etching, Dental; Dental Enamel; Dental Fissures; Dental Leakage; Female; Humans; Laser Therapy; Male; Molar, Third; Phosphoric Acids; Pit and Fissure Sealants; Surface Properties
PubMed: 24308402
DOI: 10.5644/ama2006-124.90 -
The Korean Journal of Physiology &... Mar 2021Coronary microembolization (CME) is associated with cardiomyocyte apoptosis and cardiac dysfunction. Puerarin confers protection against multiple cardiovascular...
Coronary microembolization (CME) is associated with cardiomyocyte apoptosis and cardiac dysfunction. Puerarin confers protection against multiple cardiovascular diseases, but its effects and specific mechanisms on CME are not fully known. Hence, our study investigated whether puerarin pretreatment could alleviate cardiomyocyte apoptosis and improve cardiac function following CME. The molecular mechanism associated was also explored. A total of 48 Sprague-Dawley rats were randomly divided into CME, CME + Puerarin (CME + Pue), sham, and sham + Puerarin (sham + Pue) groups (with 12 rats per group). A CME model was established in CME and CME + Pue groups by injecting 42 μm microspheres into the left ventricle of rats. Rats in the CME + Pue and sham + Pue groups were intraperitoneally injected with puerarin at 120 mg/kg daily for 7 days before operation. Cardiac function, myocardial histopathology, and cardiomyocyte apoptosis index were determined cardiac ultrasound, hematoxylin-eosin (H&E) and hematoxylin-basic fuchsin-picric acid (HBFP) stainings, and TdT-mediated dUTP nick-end labeling (TUNEL) staining, respectively. Western blotting was used to measure protein expression related to the phosphoinositide 3-kinase (PI3K)/protein kinase B (Akt)/glycogen synthase kinase-3β (GSK-3β) pathway. We found that, puerarin significantly ameliorated cardiac dysfunction after CME, attenuated myocardial infarct size, and reduced myocardial apoptotic index. Besides, puerarin inhibited cardiomyocyte apoptosis, as revealed by decreased Bax and cleaved caspase-3, and up-regulated Bcl-2 and PI3K/Akt/GSK-3β pathway related proteins. Collectively, puerarin can inhibit cardiomyocyte apoptosis and thus attenuate myocardial injury caused by CME. Mechanistically, these effects may be achieved through activation of the PI3K/Akt/GSK-3β pathway.
PubMed: 33602885
DOI: 10.4196/kjpp.2021.25.2.147 -
International Journal of Dentistry 2020Secondary caries originate from a leakage pathway where oral acids can penetrate faster and demineralize the tooth substrate deeper which can be visualized by dye...
PURPOSE
Secondary caries originate from a leakage pathway where oral acids can penetrate faster and demineralize the tooth substrate deeper which can be visualized by dye penetration. The ability to prevent secondary caries by contemporary adhesive systems was evaluated in this study. Dye penetration distance through leakage and into the tooth substrate adjacent to Class V restorations after artificial caries exposure was compared.
MATERIALS AND METHODS
Previously frozen extracted human molars were used to prepare the Class V cavities at the CEJ on axial surfaces. All cavities were restored with either the resin-composite or amalgam with or without resin adhesives: dry bonding: Super-Bond D-Liner II Plus; moist bonding: All-Bond 2; and self-etch bonding: AQ Bond and Clearfil Protect Bond. Two subgroups of Super-Bond D-Liner II Plus were immersed for 14 days at 37°C either in artificial saliva (negative control) or the artificial caries solution. The other groups were soaked in the artificial caries solution. The distance of dye penetration into the adjacent enamel, cementum/dentin, and tooth-resin interfaces was measured after immersion in 0.5% basic fuchsin dye for 24 h. The tooth-resin interfacial layer was investigated using SEM.
RESULTS
No dye penetration into the tooth-resin interface was found in Super-Bond D-Liner II Plus and AQ Bond groups which demonstrated a constant hybrid layer after a chemical challenge. The leakage distance at the cementum/dentin-resin interface of All-Bond 2, Clearfil Protect Bond, and non-adhesive amalgam (positive control) groups was significantly higher than the distance of dye penetration into the adjacent demineralized root surface ( < 0.05).
CONCLUSION
Caries associated with either amalgam or resin-composite restorations can be prevented using resin adhesives which can penetrate into the intact tooth substrate to form a stable hybrid layer. With caries-free restorations, tooth vitality may be conserved lifelong.
PubMed: 32774375
DOI: 10.1155/2020/5859835 -
Zhong Nan Da Xue Xue Bao. Yi Xue Ban =... Aug 2023Laryngeal cancer (LC) is a globally prevalent and highly lethal tumor. Despite extensive efforts, the underlying mechanisms of LC remain inadequately understood. This...
OBJECTIVES
Laryngeal cancer (LC) is a globally prevalent and highly lethal tumor. Despite extensive efforts, the underlying mechanisms of LC remain inadequately understood. This study aims to conduct an innovative bioinformatic analysis to identify hub genes that could potentially serve as biomarkers or therapeutic targets in LC.
METHODS
We acquired a dataset consisting of 117 LC patient samples, 16 746 LC gene RNA sequencing data points, and 9 clinical features from the Cancer Genome Atlas (TCGA) database in the United States. We employed weighted gene co-expression network analysis (WGCNA) to construct multiple co-expression gene modules. Subsequently, we assessed the correlations between these co-expression modules and clinical features to validate their associations. We also explored the interplay between modules to identify pivotal genes within disease pathways. Finally, we used the Kaplan-Meier plotter to validate the correlation between enriched genes and LC prognosis.
RESULTS
WGCNA analysis led to the creation of a total of 16 co-expression gene modules related to LC. Four of these modules (designated as the yellow, magenta, black, and brown modules) exhibited significant correlations with 3 clinical features: The age of initial pathological diagnosis, cancer status, and pathological N stage. Specifically, the yellow and magenta gene modules displayed negative correlations with the age of pathological diagnosis (=-0.23, <0.05; =-0.33, <0.05), while the black and brown gene modules demonstrated negative associations with cancer status (=-0.39, <0.05; =-0.50, <0.05). The brown gene module displayed a positive correlation with pathological N stage. Gene Ontology (GO) enrichment analysis identified 77 items, and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis identified 30 related signaling pathways, including the calcium signaling pathway, cytokine-cytokine receptor interaction, neuro active ligand-receptor interaction, and regulation of lipolysis in adipocytes, etc. Consequently, central genes within these modules that were significantly linked to the overall survival rate of LC patients were identified. Central genes included , , , , , , , and .
CONCLUSIONS
This study, utilizing WGCNA and subsequent validation, pinpointed 8 genes with potential as gene biomarkers for LC. These findings offer valuable references for the clinical diagnosis, prognosis, and treatment of LC.
Topics: Humans; Laryngeal Neoplasms; Rosaniline Dyes; Biomarkers; Adipocytes; Gene Regulatory Networks; Gene Expression Profiling
PubMed: 37875354
DOI: 10.11817/j.issn.1672-7347.2023.220630